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Figure 6 | Journal of Molecular Signaling

Figure 6

From: Breast cancer cell invasion mediated by Gα12 signaling involves expression of interleukins-6 and −8, and matrix metalloproteinase-2

Figure 6

Ectopic expression of dominant active Gα12 in MDA-MB-231 cells enhances promoter activities of IL-6, IL-8 and MMP-2. Luciferase-based assays were conducted to analyze the impact of Gα12QL expression on the activities of an IL-6-Luc promoter (A), IL-8-Luc promoter construct (B), and a MMP-2-Luc promoter construct. (C) Mock (M) or Gα12QL expression vectors (200 ng of DNA per well of a 24-well plate) were co-transfected into the MDA-MB-231 cells along with the IL-6-Luc, IL-8-Luc or MMP-2-Luc vectors respectively, as well as pRL-TK (renilla control) as described in Methods. Induction of promoter activity by dominant active Gα12 expression or 10 ng/ml PMA (positive control) was assessed for the respective constructs, corrected for transfection efficiency, and calculated as percentage of induction compared with unstimulated baseline activity. Data shown were pooled from three independent experiments, each with triplicate determinations. Bars represent the mean ± S.E.

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Journal of Molecular Signaling

ISSN: 1750-2187