Stage dependent expression of the different PI3K Type IA catalytic isoforms.
A) Protein levels of the different PI3K Type IA catalytical subunits were analyzed with western blot using two sets of primary neuroblastoma tumors. Abbreviations: AG, adrenal gland; MNA, MYCN amplification B) Relative quantification (RQ) of p110α, β and δ protein levels in primary tumors. Boxplot explanation; upper hinge of the box, 75th percentile; lower hinge of the box, 25th percentile; thick horizontal line within box, median. The whiskers are indicating range, open circles represent outliers while asterisks represent extremes. C) Western blot of cell lines with different origin show that cell lines of neuroblastic origin has high levels of p110α and low levels of p110β compared to non neuroblastic cell lines. Grading of MNA; no gain or amplification (−), gain (+), amplified (++).