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Table 1 Photobleaching time-constants and relative-FRET efficiencies in HEK-293 cells coexpressing SSTR2 and SSTR3

From: Coexpression of human somatostatin receptor-2 (SSTR2) and SSTR3 modulates antiproliferative signaling and apoptosis

Treatment τ avg (s) n E (%)
Control D - A 18.3 ± 0.5 55 14.9 ± 1.2
D + A 21.5 ± 0.7 54
SST (1 nM) D - A 18.6 ± 0.4 58 11.4 ± 1.4*
D + A 21 ± 0.5 56
SST (50 nM) D - A 18.8 ± 0.4 59 12.1 ± 1.1
D + A 21.4 ± 0.4 60
SST (1 μM) D - A 19.6 ± 0.6 57 10.9 ± 1.6*
D + A 22 ± 0.2 55
L-779976 (10 nM) D - A 20.7 ± 0.3 56 10.4 ± 1.5*
D + A 23.1 ± 0.3 59
L-779976 (100 nM) D - A 20.3 ± 0.4 58 12.1 ± 1.2
D + A 23.1 ± 0.4 54
L-796778 (25 nM) D – A 19.5 ± 0.9 59 12.9 ± 1.7
D + A 22.4 ± 0.7 54
L-796778 (50 nM) D - A 20.8 ± 0.5 60 8 ± 1.2*
D + A 22.6 ± 0.6 57
L-779976 (10 nM) + L-796778 (25 nM) D - A 19.6 ± 0.5 55 11.3 ± 0.9*
D + A 22.1 ± 0.2 54
L-779976 (100 nM) + L-796778 (25 nM) D - A 19.6 ± 0.5 57 12.9 ± 1.4
D + A 22.5 ± 0.5 54
  1. D − A and D + A correspond to donor without and with acceptor, respectively; τ avg refers to average of n photobleaching time constants; n is the total number of cells analyzed; E (%) is mean relative FRET efficiency. Mean ± SD is representative of three independent experiments (*, p < 0.05).