Coexpression of human somatostatin receptor-2 (SSTR2) and SSTR3 modulates antiproliferative signaling and apoptosis

Table 1 Photobleaching time-constants and relative-FRET efficiencies in HEK-293 cells coexpressing SSTR2 and SSTR3

Treatment	τ _avg (s)		n	E (%)
Control	D - A	18.3 ± 0.5	55	14.9 ± 1.2
Control	D + A	21.5 ± 0.7	54	14.9 ± 1.2
SST (1 nM)	D - A	18.6 ± 0.4	58	11.4 ± 1.4*
SST (1 nM)	D + A	21 ± 0.5	56	11.4 ± 1.4*
SST (50 nM)	D - A	18.8 ± 0.4	59	12.1 ± 1.1
SST (50 nM)	D + A	21.4 ± 0.4	60	12.1 ± 1.1
SST (1 μM)	D - A	19.6 ± 0.6	57	10.9 ± 1.6*
SST (1 μM)	D + A	22 ± 0.2	55	10.9 ± 1.6*
L-779976 (10 nM)	D - A	20.7 ± 0.3	56	10.4 ± 1.5*
L-779976 (10 nM)	D + A	23.1 ± 0.3	59	10.4 ± 1.5*
L-779976 (100 nM)	D - A	20.3 ± 0.4	58	12.1 ± 1.2
L-779976 (100 nM)	D + A	23.1 ± 0.4	54	12.1 ± 1.2
L-796778 (25 nM)	D – A	19.5 ± 0.9	59	12.9 ± 1.7
L-796778 (25 nM)	D + A	22.4 ± 0.7	54	12.9 ± 1.7
L-796778 (50 nM)	D - A	20.8 ± 0.5	60	8 ± 1.2*
L-796778 (50 nM)	D + A	22.6 ± 0.6	57	8 ± 1.2*
L-779976 (10 nM) + L-796778 (25 nM)	D - A	19.6 ± 0.5	55	11.3 ± 0.9*
L-779976 (10 nM) + L-796778 (25 nM)	D + A	22.1 ± 0.2	54	11.3 ± 0.9*
L-779976 (100 nM) + L-796778 (25 nM)	D - A	19.6 ± 0.5	57	12.9 ± 1.4
L-779976 (100 nM) + L-796778 (25 nM)	D + A	22.5 ± 0.5	54	12.9 ± 1.4

D − A and D + A correspond to donor without and with acceptor, respectively; τ _avg refers to average of n photobleaching time constants; n is the total number of cells analyzed; E (%) is mean relative FRET efficiency. Mean ± SD is representative of three independent experiments (*, p < 0.05).