|
Treatment
|
τ
avg
(s)
|
n
|
E (%)
|
|---|
|
Control
|
D - A
|
18.3 ± 0.5
|
55
|
14.9 ± 1.2
|
|
D + A
|
21.5 ± 0.7
|
54
|
|
SST (1 nM)
|
D - A
|
18.6 ± 0.4
|
58
|
11.4 ± 1.4*
|
|
D + A
|
21 ± 0.5
|
56
|
|
SST (50 nM)
|
D - A
|
18.8 ± 0.4
|
59
|
12.1 ± 1.1
|
|
D + A
|
21.4 ± 0.4
|
60
|
|
SST (1 μM)
|
D - A
|
19.6 ± 0.6
|
57
|
10.9 ± 1.6*
|
|
D + A
|
22 ± 0.2
|
55
|
|
L-779976 (10 nM)
|
D - A
|
20.7 ± 0.3
|
56
|
10.4 ± 1.5*
|
|
D + A
|
23.1 ± 0.3
|
59
|
|
L-779976 (100 nM)
|
D - A
|
20.3 ± 0.4
|
58
|
12.1 ± 1.2
|
|
D + A
|
23.1 ± 0.4
|
54
|
|
L-796778 (25 nM)
|
D – A
|
19.5 ± 0.9
|
59
|
12.9 ± 1.7
|
|
D + A
|
22.4 ± 0.7
|
54
|
|
L-796778 (50 nM)
|
D - A
|
20.8 ± 0.5
|
60
|
8 ± 1.2*
|
|
D + A
|
22.6 ± 0.6
|
57
|
|
L-779976 (10 nM) + L-796778 (25 nM)
|
D - A
|
19.6 ± 0.5
|
55
|
11.3 ± 0.9*
|
|
D + A
|
22.1 ± 0.2
|
54
|
|
L-779976 (100 nM) + L-796778 (25 nM)
|
D - A
|
19.6 ± 0.5
|
57
|
12.9 ± 1.4
|
|
D + A
|
22.5 ± 0.5
|
54
|
- D − A and D + A correspond to donor without and with acceptor, respectively; τ
avg
refers to average of n photobleaching time constants; n is the total number of cells analyzed; E (%) is mean relative FRET efficiency. Mean ± SD is representative of three independent experiments (*, p < 0.05).
