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Table 1 Photobleaching time-constants and relative-FRET efficiencies in HEK-293 cells coexpressing SSTR2 and SSTR3

From: Coexpression of human somatostatin receptor-2 (SSTR2) and SSTR3 modulates antiproliferative signaling and apoptosis

Treatment

τ avg (s)

n

E (%)

Control

D - A

18.3 ± 0.5

55

14.9 ± 1.2

D + A

21.5 ± 0.7

54

SST (1 nM)

D - A

18.6 ± 0.4

58

11.4 ± 1.4*

D + A

21 ± 0.5

56

SST (50 nM)

D - A

18.8 ± 0.4

59

12.1 ± 1.1

D + A

21.4 ± 0.4

60

SST (1 μM)

D - A

19.6 ± 0.6

57

10.9 ± 1.6*

D + A

22 ± 0.2

55

L-779976 (10 nM)

D - A

20.7 ± 0.3

56

10.4 ± 1.5*

D + A

23.1 ± 0.3

59

L-779976 (100 nM)

D - A

20.3 ± 0.4

58

12.1 ± 1.2

D + A

23.1 ± 0.4

54

L-796778 (25 nM)

D – A

19.5 ± 0.9

59

12.9 ± 1.7

D + A

22.4 ± 0.7

54

L-796778 (50 nM)

D - A

20.8 ± 0.5

60

8 ± 1.2*

D + A

22.6 ± 0.6

57

L-779976 (10 nM) + L-796778 (25 nM)

D - A

19.6 ± 0.5

55

11.3 ± 0.9*

D + A

22.1 ± 0.2

54

L-779976 (100 nM) + L-796778 (25 nM)

D - A

19.6 ± 0.5

57

12.9 ± 1.4

D + A

22.5 ± 0.5

54

  1. D − A and D + A correspond to donor without and with acceptor, respectively; τ avg refers to average of n photobleaching time constants; n is the total number of cells analyzed; E (%) is mean relative FRET efficiency. Mean ± SD is representative of three independent experiments (*, p < 0.05).