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Figure 9 | Journal of Molecular Signaling

Figure 9

From: Heterodimerization of β2 adrenergic receptor and somatostatin receptor 5: Implications in modulation of signaling pathway

Figure 9

hSSTR5 and β 2 AR blocks the dephosphorylation of NFAT. Mono-and cotransfected HEK-293 cells were treated with SST (1 μM), L-817818 (10 nM), β2 agonist (1 μM) alone or in combination at 37°C for 30 min and analyzed for total and phosphorylated NFAT in presence of basal and elevated Ca2+. In the presence of basal Ca2+ no significant changes were seen in NFAT phosphorylation in mono-and/or cotransfected cells expressing β2AR or hSSTR5 (Panel A). In the presence of high Ca2+ in culture medium, increased level of phosphorylated NFAT was observed in monotransfected cells when compared with basal Ca2+ control. Cotransfected cells displayed increased phospho-NFAT upon formoterol treatment whereas NFAT phosphorylation remained comparable to control upon activation of SSTR5 alone or in combination with β2AR agonist (Panel B). Densitometric analysis of western blot for pNFAT is shown in panels C and D. Data analysis was done by using ANOVA and post hoc Dunnett's to compare against control (*, p < 0.05).

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Journal of Molecular Signaling

ISSN: 1750-2187