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Figure 7 | Journal of Molecular Signaling

Figure 7

From: Heterodimerization of β2 adrenergic receptor and somatostatin receptor 5: Implications in modulation of signaling pathway

Figure 7

Time and receptor specific changes in PKA phosphorylation. To determine the expression levels of total and phospho-PKA, mono-and cotransfected cells were treated with SST, L-817818 and formoterol alone or in combination for 10 and 30 min at 37°C in the presence of basal (Panels A and B) and higher Ca2+ (Panel C). Upon 10 min treatment, cells expressing hSSTR5 displayed inhibition of PKA phosphorylation in the presence of SST whereas no significant phosphorylation of PKA was detected in cells expressing β2AR. In cotransfected cells, β2AR effect was predominant showing inhibition of pPKA with all treatments as indicated (Panel A). Interestingly, PKA phosphorylation was enhanced in SSTR5 monotransfectant in control or in presence of SST for 30 min. With high Ca2+ in culture medium, pPKA expression was higher in the presence of β2AR in comparison to hSSTR5 and was inhibited upon activation of receptors respectively. In cotransfected cells, enhanced PKA phosphorylation seen in monotransfected cells was completely diminished following agonist treatment (Panel C). Densitometric analysis of western blot for pPKA is shown. Data analysis was done by using ANOVA and post hoc Dunnett's to compare against basal level (*, p < 0.01).

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Journal of Molecular Signaling

ISSN: 1750-2187