Figure 2

Expression levels of MK2, MK3, and MK5 in HEK293 cells. (A) Total RNA was reverse transcribed and MK2, MK3, MK5, and rRNA transcripts were amplified by PCR with specific primers. PCR products were visualized by electrophoresis on an agarose gel in the presence of ethidium bromide. A size marker (in base-pairs; bp) was run on the gel to confirm the correct size of the PCR products. rRNA was used as an internal control [33]. (B) The relative amounts of MK2, MK3, and MK5 in HEK293 cells was determined by western blotting. The band corresponding to the molecular mass of MK2, MK3, and MK5 is indicated by an arrow. To assure equal sample loading, the blot was stripped and re-probed with antibodies against actin. The molecular mass (in kD) of a protein marker is shown.