Figure 3

Oligomerization of AKAP12 (and either C-terminal or N-terminal fragments) expressed in mammalian HEK293 cells. Human embryonic kidney (HEK) 293 cells were transfected with an empty expression vector or one harboring either full-length AKAP12, AKAP12 N-terminal (1-938) or C-terminal (840-1782) regions. Whole-cell lysates of cells expressing either AKAP12 or one of the fragments were subjected to SDS-PAGE in the absence (A) or presence of 8 M urea (B). The resolved proteins were transferred to PVDF membrane and subjected to immunoblotting, stained for AKAP12 or the HA-tag. The results displayed are representative of at least three separate experiments performed on as many separate cell cultures.