Figure 2
Steric-exclusion chromatography of purified AKAP12. His-tagged AKAP12 was purified and then resolved by steric-exclusion chromatography performed on an AKTA FPLC fitted with HiPrep Sephacryl-S400 (16/60) column. The presence of supermolecular oligomers of AKAP12 was established by SDS-PAGE and immunoblotting of samples from the chromatography. The resolved, transferred proteins were stained with anti-AKAP12 antibodies. Marker protein mobilities were employed to establish the M r of AKAP12 by elution position (inset). The results displayed are representative of three separate experiments performed on as many separate cell cultures.