Figure 3
From: Activation of K-RAS by co-mutation of codons 19 and 20 is transforming
Quantification of focus formation by different K-RAS mutations. Histogram of mean focus numbers obtained from focus formation assays performed in 4 separate experiments after NIH3T3 cells were transfected with K-RAS cDNA expression vectors containing G12V, G12D, L19F, T20A, L19F/T20A or wildtype K-RAS sequences. Error bars represent ±SD. Full focus counts for all transfections and all experiments and the sequences of the cDNA constructs used for NIH3T3 cell transfection are described in Additional File section (Additional File 1).