Figure 3

Mass spectrometry-based proteomic analysis enables identification of the proteins that constitute large supermolecular complexes such as Dvl3-based signalsomes. The ability to fragment and subsequently sequence a complex of proteins, such as the Dvl3-based signalsome, is essential for similar efforts aimed at establishing the identity of protein partners involved in large complexes that integrate and propagate signaling downstream. These mass spectrometry-based analyses can be performed in parallel or sequentially to optimize the discovery of constituent peptides.