5 MDa of the Dvl3-based signalsome. Fluorescence correlation spectroscopy (fcs) is a single molecule analysis performed in live cells that experimentally measures the diffusion coefficient and permits calculation of MW of the signalsome (0.2 and 30 MDa species in vivo), which also reveal an upfield shift in MW in response to Wnt3a. Proteomics provides for molecular dissection of the composition of the signalsome isolated from untreated and Wnt3a-treated cells. Conclusion Dvl3-based punctae/signalsomes made visible by fluorescent microscopy now can be interrogated by advanced physical means, defining such properties as signalsome M r /MW, molecular composition, and intracellular locale."/>
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Figure 3 | Journal of Molecular Signaling

Figure 3

From: Probing the physical nature and composition of signalsomes

Figure 3

Mass spectrometry-based proteomic analysis enables identification of the proteins that constitute large supermolecular complexes such as Dvl3-based signalsomes. The ability to fragment and subsequently sequence a complex of proteins, such as the Dvl3-based signalsome, is essential for similar efforts aimed at establishing the identity of protein partners involved in large complexes that integrate and propagate signaling downstream. These mass spectrometry-based analyses can be performed in parallel or sequentially to optimize the discovery of constituent peptides.

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