Figure 4
From: A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1
CARP-1 (651-759) binds with TAZ (1-120). Schematic diagram of CARP-1 (WT and its various mutants; Left Box) and TAZ (WT and its mutants; Right Box) constructs that were utilized to map minimal epitopes for their interaction. All CARP-1 proteins have myc and 6 × His epitopes at their carboxyl termini. All the TAZ proteins, with the exception of 1-120, 1-164, and 165-395, harbored flag epitope at their amino termini, while TAZ 1-120 and its 1-164 and 165-395 mutants had Gst epitope at their amino termini. Positive interactions are indicated by + and loss/absence of interaction is denoted by -.