Figure 1

H89 attenuates cell proliferation (A) and enhances apoptosis (B). Cells growing in normal serum conditions were either untreated (noted as 0) or treated with noted doses of H89 for indicated time, followed by determination of cell proliferation by MTT assay (panel A) or apoptosis (panel B) as in methods. Columns in histograms in panels A and B represent data from 3 independent experiments; bars, SE. Data in both panels were analyzed using a two-tailed Student t test. For panel A, *P and **P < 0.0018 and 0.00002, respectively, and for panel B, *P and **P < 0.000026 and 0.0003, respectively, compared to the corresponding untreated controls.