Figure 7

Effect of Akt inhibition on TRG-induced apoptosis resistance in serum-containing media. (A) Subconfluent Huh-7 cells were transfected with either control-siRNA (lanes 1 & 2), AKT-siRNA (lanes 3 & 4), or Akt-3m-siRNA (lanes 5 & 6) for 72 hours followed by treatment with 25 μM TRG for 24 hours in serum-containing media. Western Blot analysis was then performed with the antibodies indicated. (B) MEFs from Wild type (lanes 1 & 2), Akt1 KO (lanes 3 & 4) or Akt1/2 KO (lanes 5 & 6) mice were treated with 25 μM TRG in serum-containing media for 24 hours followed by Western Blot analysis. TRG-treated Huh-7 cell extracts in serum-deficient media were used as positive controls for PARP and Caspase-3 cleavage in A & B and WT-MEF extract as positive control for Akt3 in A.