Skip to main content
Figure 4 | Journal of Molecular Signaling

Figure 4

From: Peroxisome proliferator-activated receptor gamma ligand-mediated apoptosis of hepatocellular carcinoma cells depends upon modulation of PI3Kinase pathway independent of Akt

Figure 4

Effect of TRG on PI3K/Akt Pathway in the presence or absence of serum. (A) Huh-7 cells were treated with 25 μM TRG in the absence (-) or presence (+) of serum for the indicated periods of time. Equal amounts of cell extracts were analyzed by Western Blot analysis utilizing antibodies against pAktSer473, Akt or GAPDH. (B) Western Blot analysis of the cell extracts treated with TRG in serum-deficient media following normalization of total AKT levels. (C) Cells treated with increasing concentrations of TRG for 6 hours in serum deficient media were subjected to Western Blot analysis as in A.

Back to article page

Journal of Molecular Signaling

ISSN: 1750-2187