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Figure 2 | Journal of Molecular Signaling

Figure 2

From: Peroxisome proliferator-activated receptor gamma ligand-mediated apoptosis of hepatocellular carcinoma cells depends upon modulation of PI3Kinase pathway independent of Akt

Figure 2

Role of PI3Kinase pathway on TRG-induced cell growth arrest. (A) Subconfluent Huh-7 cells were treated as in 1B or (B) as in 1D, followed by Western Blot analysis of the cell extracts with antibodies against pAktSer473, Akt and GAPDH. (C) Huh-7 cells were treated in the absence (-) or presence (+) of 25 μM TRG for 24 hours following a 1 hour pretreatment with either none (lanes 1 & 2), or 1 μM Wortmannin (lanes 3 & 4) or 5 μM LY294002 (lanes 5 & 6). Western Blot analysis was then performed with the antibodies indicated. (D) Subconfluent Huh-7 cells were treated with DMSO, 25 μM TRG, DMSO + 1 μM Wortmannin, or TRG + 1 μM Wortmannin for the indicated time intervals, following a 1 hour pretreatment with Wortmannin. Cell proliferation assay was performed as described in 1A.

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Journal of Molecular Signaling

ISSN: 1750-2187