Dishevelled-3 C-terminal His single amino acid repeats are obligate for Wnt5a activation of non-canonical signaling

Table 1 Comparison of His mutants of hDvl3 in the ability of rescuing Wnt non-canonical signaling.

F9 cells expressing β₂AR/Fz2 were treated with siRNA targeting Dvl3 (siRNA). Rescue experiments were conducted by expression of hDvl3, or hDvl3 mutants containing His to Ala point mutation as indicated. The non-canonical pathway was activated by treating cells with or without isoproterenol (10 μM). Activity of NF-AT-reporter was measured and analyzed, setting the full rescue by hDvl3 as 100%.