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Figure 2 | Journal of Molecular Signaling

Figure 2

From: Dishevelled-3 C-terminal His single amino acid repeats are obligate for Wnt5a activation of non-canonical signaling

Figure 2

Dvl3 is requisite in Wnt5a/Fz2/cGMP/Ca 2+ signaling. A , Fz2 expressing cells were treated with control siRNA (-) or siRNA targeting individual Dvl isoforms. Cells were treated with vehicle (-Wnt5a), or Wnt5a (+Wnt5a) for 45 min and cellular cGMP was assayed (left panel). Suppression of Dvl1, 2 or 3 by using siRNA was examined by immunoblotting (right panel). B, cells were treated for 48 hr with either control siRNA (Ctr) or siRNA targeting individual Dvl isoforms. After loaded with Fura2, cells were challenged by Wnt5a (+Wnt5a). A representative graph of Ca2+ transient presented as A340/A380 for cells treated with different siRNA presented. Each line depicts the mean values obtained from 20 cells selected randomly.

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Journal of Molecular Signaling

ISSN: 1750-2187