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Figure 5 | Journal of Molecular Signaling

Figure 5

From: AMPK-induced activation of Akt by AICAR is mediated by IGF-1R dependent and independent mechanisms in acute lymphoblastic leukemia

Figure 5

Expression level of IGF-1R and downstream regulated signaling factors in B-ALL vs . T-ALL cell lines. A) Western blot analysis of P-IGF-1R (Tyr1131), P-IRS-1 (Ser794 and Ser312), and P-4EBP1 (Thr70) expression in Bp-ALL (NALM6) and T-ALL cells (CCRF-CEM) treated with 0.1% DMSO (Control) or the IGF-1R inhibitor HNMPA(AM)3 (IGF1Ri, 10 μM) and incubated for 24 h. B) Basal expression level of P-IGF-1R (Tyr1131), P-IRS-1 (Ser312), P-Akt (Thr308), and P-4EBP1 (Thr70) in Bp-ALL NALM6 and subtypes characterized by non-random translocations (REH (t[12;21]), and SupB15 (t[9;22])). The levels of P-4EBP1 were normalized to β-actin (loading control) and expressed relative to control (shown as fold induction).

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Journal of Molecular Signaling

ISSN: 1750-2187