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Figure 3 | Journal of Molecular Signaling

Figure 3

From: Novel cis-trans interactions are involved in post-transcriptional regulation of cyclin-dependent kinase inhibitor p21WAF1/CIP1 mRNA

Figure 3

CD437 targets 3'-UTR sequences of p21 WAF1/CIP1 mRNA independent of their location in the chimeric RBG transcripts. (A) Two independent sublines derived from stable transfection of each of the RBG-WAF (1540-1700) plasmid (labelled as A5 and A6) and WAF (1540-1700)-RBG plasmid (labeled as 1, 2) were grown either in the absence (-) or presence (+) of 1 μM CD437 for 48 hours. The cellular RNAs were prepared and northern blot hybridizations carried out as described in the legend to figure 1. (B) Histogram showing relative expression of chimeric RBG-WAF transcripts in control vs CD437-treated sublines in panel A. Northern blots of panel A were quantitated by soft laser densitometry. Columns represent mRNA levels relative to the levels of mRNAs present in the untreated control lanes, which were arbitrarily defined as 1. The error bars represent the standard error of the mean.

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Journal of Molecular Signaling

ISSN: 1750-2187