Skip to main content
Figure 2 | Journal of Molecular Signaling

Figure 2

From: Novel cis-trans interactions are involved in post-transcriptional regulation of cyclin-dependent kinase inhibitor p21WAF1/CIP1 mRNA

Figure 2

Presence of p21 WAF1/CIP1 3'-UTR causes destabilization of RBG mRNA, while CD437 treatments enhance stability of chimeric RBG-WAF transcripts. MDA-MB-468 sublines derived from stable transfection of RBG-WAF (581-2004) plasmid (Panel A) or RBG plasmid (not shown) were cultured in the absence (-) or presence (+) of CD437 for 48 hours and exposed to transcriptional inhibitor ACT D for noted times. The preparation of RNA and northern blot hybridization were as described in the legend to figure 1. Panels B and C show plots of the decay of RBG transcripts in either the presence or absence of CD437. The RBG mRNA levels at time 0 in either the absence or presence of CD437 were arbitrarily defined as 1. Data in panels B and C represent mean of four and two independent sublines, respectively, for each time point. Error bars represent the standard error of the mean.

Back to article page

Journal of Molecular Signaling

ISSN: 1750-2187