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Figure 8 | Journal of Molecular Signaling

Figure 8

From: Immature and mature species of the human Prostacyclin Receptor are ubiquitinated and targeted to the 26S proteasomal or lysosomal degradation pathways, respectively

Figure 8

Effect of MG132 on radioligand binding by the hIP. HEK.hIP cells were pretreated with DMSO (0.001%) or MG132 (10 μM) for 0-12 hr, with untreated cells serving as the reference. Cells were harvested and radioligand-binding assays were then performed on crude membrane (P100) fractions in the presence of 4 nM [3H]iloprost at 30°C, as outlined in Experimental Procedures. The data are presented as the mean [3H]iloprost bound (pmol/mg protein ± S.E.M., n = 4). For untreated cells (0 hr), the actual value for mean [3H]iloprost bound (pmol/mg protein ± S.E.M.) was 1.55 ± 0.10 pmol/mg protein. ***, p < 0.0001 indicates that the mean [3H]iloprost bound (pmol/mg protein) ± S.E.M. was significantly reduced in the presence of MG132 compared to DMSO at each time point.

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Journal of Molecular Signaling

ISSN: 1750-2187