Skip to main content
Figure 7 | Journal of Molecular Signaling

Figure 7

From: Immature and mature species of the human Prostacyclin Receptor are ubiquitinated and targeted to the 26S proteasomal or lysosomal degradation pathways, respectively

Figure 7

Subcellular localisation of the hIP in the presence of MG132. HEK.hIP cells were pretreated in the presence (+) or absence (-) of vehicle (0.001% DMSO, lanes 1-3) or MG132 (10 μM, lanes 4-6) for 12 hr. Aliquots of total (T; 50 μg), membrane (P100; 37.5 μg) and soluble (S100; 25 μg) fractions were resolved by SDS-PAGE, electroblotted onto PVDF membrane and screened with the anti-HA (3F10) antibody. The arrows at the right of the panel indicate the core glycosylated, non-farnesylated (44 kDa); core glycosylated, farnesylated (42 kDa); non-glycosylated, non-farnesylated (40 kDa); and non-glycosylated, farnesylated (38 kDa) species of the hIP, respectively, that accumulate following the various treatments. The positions of the molecular size markers (kDa) are indicated to the left of the panel. Data are representative of three independent experiments.

Back to article page

Journal of Molecular Signaling

ISSN: 1750-2187