Figure 8

Both AKT and eNOS inhibitors prolong and augment Erk1/2 phosphorylation. GH was administrated first at 0 min and a second time at 75 min to cell cultures with or without AKT inhibitor pre-treatment. Whole cell extracts were prepared. A) Western blots using an antibody against phospho-Erk1/2 and Erk1/2 show that the phosphorylation of Erk1/2 starts 15 min after the first GH administration and 2 levels off after 75 min even with secondary GH administration. B) For comparison, cell extracts from cells with single GH stimulation were prepared and Western blots were performed for Erk1/2 and phosphorylated Erk1/2. C) Quantitation of the Western blots data. *: First GH challenge, **: second GH challenge. AKTi: Akt inhibitor, eNOSi: eNOS inhibitor.