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Figure 9 | Journal of Molecular Signaling

Figure 9

From: Src homology 2 (SH2) domain containing protein tyrosine phosphatase-1 (SHP-1) dephosphorylates VEGF Receptor-2 and attenuates endothelial DNA synthesis, but not migration*

Figure 9

Effect of SHP-1 mutations on de-phosphorylation of tyrosine residues on KDR. (A) HUVEC was transfected with the following plasmids control pCDNA (C) or SHP-1 wildtype (WT) or SHP-1 Y538 or SHP-1 Y543 or SHP-1 Y566 mutants using nucleofector based electroporation. After 48 hrs. the cells were starved overnight in EBM medium without serum. Subsequently the cells were stimulated with VEGF for 5 min. Cell lysates were collected and expression of SHP-1 was confirmed. The same lysates were immunoblotted with antibodies against p-Tyr 1059 and p-Tyr 1175 of KDR. Total KDr and β-actin levels were also determined. NIH Image quantitation data of (B) fold change in Tyr 1059 and (C) fold change in Tyr 1175 of KDR normalized with respect to total KDR. A representative image is shown the experiment was repeated three times.

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Journal of Molecular Signaling

ISSN: 1750-2187