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Figure 7 | Journal of Molecular Signaling

Figure 7

From: Src homology 2 (SH2) domain containing protein tyrosine phosphatase-1 (SHP-1) dephosphorylates VEGF Receptor-2 and attenuates endothelial DNA synthesis, but not migration*

Figure 7

Effect of inhibition of c-Src on phospho-Tyr of KDR and DNA synthesis. (A) HUVEC was pretreated with PP2 or PP3 at 2.5 or 5 uM for 1 h and then with or without VEGF 10 ng/ml and immunoblotted with antibodies against p-Tyr of KDR. Total KDR served as loading control. (B) HUVEC were plated in 24 well plates, pretreated with PP2 or PP3 at 5 uM for 1 h and then with or without VEGF 10 ng/ml and [3H]thymidine incorporation carried out as described in Materials and Methods. Another set of the same treatment was subjected to a western blot for pERK and total ERK. Data represent average of three independent determinations each in triplicate (P = 0.03).

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Journal of Molecular Signaling

ISSN: 1750-2187