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Figure 5 | Journal of Molecular Signaling

Figure 5

From: Src homology 2 (SH2) domain containing protein tyrosine phosphatase-1 (SHP-1) dephosphorylates VEGF Receptor-2 and attenuates endothelial DNA synthesis, but not migration*

Figure 5

Effect of SHP-1 knockdown on phosphorylation of KDR and ERK. (A) HUVEC were transfected with scrambled control or SHP-1 siRNA using oligofectamine. After 48 h the cells were serum starved and treated with or without VEGF 10 ng/ml and immunoblotted with antibodies against p-Tyr of KDR. Total KDR served as a loading control. (B) HUVEC were transfected with scrambled control or SHP-1 siRNA using oligofectamine. After 48 h the cells were serum starved and treated with or without VEGF 10 ng/ml and immunoblotted with antibodies against p-ERK. Total ERK served as a loading control.

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Journal of Molecular Signaling

ISSN: 1750-2187