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Figure 4 | Journal of Molecular Signaling

Figure 4

From: Src homology 2 (SH2) domain containing protein tyrosine phosphatase-1 (SHP-1) dephosphorylates VEGF Receptor-2 and attenuates endothelial DNA synthesis, but not migration*

Figure 4

Knockdown of SHP-1 using siRNA and its effect on DNA synthesis. (A) HUVEC were transfected with scrambled control or SHP-1 siRNA using oligofectamine and protein levels checked after 48 h. β actin served as a loading control. SHP-2 levels were not affected. NIH Image quantitation data normalized with respect to b actin is shown. (B) HUVEC were transfected with scrambled control or SHP-1 siRNA using oligofectamine. After 48 h, the cells were plated on 24 well plates and [3H]thymidine incorporation assay carried out as described in Materials and Methods. Data represent average of five independent determinations each in triplicate (P = 0.004).

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Journal of Molecular Signaling

ISSN: 1750-2187