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Figure 2 | Journal of Molecular Signaling

Figure 2

From: Src homology 2 (SH2) domain containing protein tyrosine phosphatase-1 (SHP-1) dephosphorylates VEGF Receptor-2 and attenuates endothelial DNA synthesis, but not migration*

Figure 2

Serine and tyrosine phosphorylation of SHP-1. (A) Serum starved HUVEC was treated with or without VEGF 10 ng/ml for 5 min and immunoprecipitated with antibody against phospho-Ser and immunoblotted with HRP conjugated SHP-1 antibody. (B) Serum starved HUVEC was pretreated with PP2 or PP3, 5 uM for 1 h followed by treatment with VEGF 10 ng/ml for 5 min and immunoprecipitated with antibody against phospho-Tyr and immunoblotted with SHP-1 and KDR antibody. Cell lysates from the same sample were also run to show total KDR levels.

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Journal of Molecular Signaling

ISSN: 1750-2187