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Figure 5 | Journal of Molecular Signaling

Figure 5

From: Inhibition of PI3K/AKT and MEK/ERK pathways act synergistically to enhance antiangiogenic effects of EGCG through activation of FOXO transcription factor

Figure 5

Phosphorylation deficient mutants of FOXO enhance EGCG-induced FOXO transcriptional activity in HUVEC cells. HUVEC cells were transiently transfected with empty vector or constructs encoding FOXO1-TM, FOXO3a-TM, or FOXO4-TM together with 6X DBE-luciferase and pRL-TK plasmids for 24 h [69]. Luciferase counts were normalized using Renilla luciferase transfection control. After transfection, cells were washed, treated with EGCG (20 μM) for 24 h, and harvested for firefly/Renilla luciferase assays using the Dual-Luciferase Reporter Assay System (Promega). Data represent the mean ± S.D. *, #, ** = significantly different from respective controls, P < 0.05.

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Journal of Molecular Signaling

ISSN: 1750-2187