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Figure 3 | Journal of Molecular Signaling

Figure 3

From: Inhibition of PI3K/AKT and MEK/ERK pathways act synergistically to enhance antiangiogenic effects of EGCG through activation of FOXO transcription factor

Figure 3

Phosphorylation deficient mutants of FOXO enhance the inhibitory effects of EGCG on migration and capillary tube formation. (A) HUVEC (4 × 104) cells were transiently transfected with empty vector, FOXO1-TM or FOXO3A-TM along with pCMV-LacZ vector (as transfection control) and treated with or without EGCG (40 μM). Migration through the membrane was determined after 24 h of incubation at 37°C. Cells that had migrated to the lower chamber were fixed with 90% ethanol, stained with Geimsa, quantified by counting the number of cells under a microscope. Data represent mean ± SD. *, ** = significantly different from control, P < 0.05. (B), HUVEC (4 × 104) cells were transiently transfected with empty vector, FOXO1-TM or FOXO3A-TM along with pCMV-LacZ vector (as transfection control) and treated with or without EGCG (40 μM) for 24 h. Capillary tubes were counted under a microscope. Data represent mean ± SD. *, ** = significantly different from control, P < 0.05.

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Journal of Molecular Signaling

ISSN: 1750-2187