Figure 4From: The α1D-adrenergic receptor is expressed intracellularly and coupled to increases in intracellular calcium and reactive oxygen species in human aortic smooth muscle cells Effect of Phenylephrine on Intracellular Calcium Levels in Stably Transfected Rat 1 Fibroblasts. Rat 1 Fibroblasts, stably transfected with each of the α1-ARs, were loaded with Calcium Green 1 AM for 1 hr. The ability of 10 uM phenylephrine to increase intracellular calcium was studied alone and following treatment with either 1 nM prazosin or 30 nM BMY 7378. Experiments were carried out as described in Methods. The results of a typical imaging study are presented along with a graphical summary of the statistical analysis of four independent experiments. Data were analyzed by a one-way ANOVA followed by post hoc testing. * Indicates a statistically significant difference from the untreated control.Back to article page