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Figure 3 | Journal of Molecular Signaling

Figure 3

From: The α1D-adrenergic receptor is expressed intracellularly and coupled to increases in intracellular calcium and reactive oxygen species in human aortic smooth muscle cells

Figure 3

Effect of Phenylephrine on Intracellular Calcium Levels in Human Aortic Smooth Muscle Cells. Human aortic smooth muscle cells were loaded with Calcium Green 1 AM for 1 hr. The ability of 25 uM phenylephrine to increase intracellular calcium was studied alone and following treatment with either 1 nM prazosin or 30 nM BMY 7378. Experiments were carried out as described in Methods. The results of a typical imaging study are presented along with a graphical summary of the statistical analysis of four independent experiments. Data were analyzed by a one-way ANOVA followed by post hoc testing. * Indicates a statistically significant difference from the untreated control.

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Journal of Molecular Signaling

ISSN: 1750-2187