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Figure 6 | Journal of Molecular Signaling

Figure 6

From: Dramatic inhibition of osteoclast sealing ring formation and bone resorption in vitro by a WASP-peptide containing pTyr294 amino acid

Figure 6

The effects of transduction of various TAT-fused WASP peptides on sealing ring formation. Osteoclasts were transduced with indicated TAT-fused peptides or treated with PBS as a control. Confocal microscopy images of osteoclasts stained for actin are shown. Sealing ring was observed in osteoclasts treated with PBS (A) or transduced with HA-TAT (B), FL-WASP (C), and HSV-TK. Sealing ring formation is reduced in osteoclasts transduced with WASP peptides consisting of BR (D), Proline-rich region (E), pTyr amino acid (F), and VC domain (G). The areas that are magnified in E' and F' are shown with a white box in Figs E and F. Arrows and arrowheads indicate small ring-like structures and actin aggregates or patches, respectively (D, E, F, E', F', and G'). Scale Bar: 50 μm. The results shown are representative of three independent osteoclast preparations and experiments.

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Journal of Molecular Signaling

ISSN: 1750-2187