Skip to main content
Figure 4 | Journal of Molecular Signaling

Figure 4

From: Dramatic inhibition of osteoclast sealing ring formation and bone resorption in vitro by a WASP-peptide containing pTyr294 amino acid

Figure 4

Analysis of the interaction of Arp2 with the endogenous WASP and transduced peptides. Osteoclasts were transduced with the WASP peptides as shown below the figures. Lysates were immunoprecipitated with a WASP (A and B) or HA (C and D) antibody. A and B: WASP immunoprecipitates were first immunoblotted with an antibody to Arp2 (A) and subsequently stripped and blotted with an antibody to WASP (B). C and D: HA-immunoprecipitates were divided into two halves; one half was immunoblotted with an Arp 2 antibody (C) and the other half was subjected to 8% (D, lane 1) and 15% (D, lanes 2–5) SDS-PAGE. Immunoblotting was performed with a HA-antibody (D) to detect the levels of transduced proteins immunoprecipitated in each lane. Immunoprecipitation with a nonimmune serum is shown in lane 6 (A-D). The results represent one of three experiments performed from three separate osteoclast preparations.

Back to article page

Journal of Molecular Signaling

ISSN: 1750-2187