Figure 2From: Dramatic inhibition of osteoclast sealing ring formation and bone resorption in vitro by a WASP-peptide containing pTyr294 amino acid Immunoblotting analysis of the levels of transduced proteins in osteoclasts with HA-antibody. Osteoclasts were transduced with the WASP peptides as shown below the figures. Osteoclast lysates (~200 μg) were immunoblotted with an antibody to HA to determine the levels of transduced proteins (Top panel). The immunoblot shown in the top panel was stripped and blotted with a GAPDH antibody for normalization (bottom panel). The results shown are representative of three independent experiments.Back to article page