Figure 5

RA increases the sensitivity of B16 cells to taxol-mediated inhibition of cell growth. B16 cells were seeded at 2.4 × 10⁴/35 mm culture dish. Following a 24-hour attachment period, cells were treated with either vehicle (DMSO) or 10 μM RA for 48 hours. After this preincubation period, the cells were refed with growth medium containing different concentrations of taxol and incubated for 16 hours. At the end of this second incubation, the cell number in each group was determined through the use of crystal violet assay, and corrected by the initial cell number seeded. The data are presented as the mean ± SEM (error bars) of triplicate dishes and are presented as percent of control (DMSO or RA pre-treated) cell number. * p < 0.05; ** p < 0.01.