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Figure 6 | Journal of Molecular Signaling

Figure 6

From: Mutations on the Switch III region and the alpha3 helix of Galpha16 differentially affect receptor coupling and regulation of downstream effectors

Figure 6

Receptor-induced activation of type II adenylyl cyclase (AC2) mediated by Gα 16 and its mutants. HEK293 cells were cotransfected with cDNAs encoding the AC2 (3 μg/ml), GαsQL (0.015 μg/ml) and fMLPR (3 μg/ml) together with Gα16, 2A, 3A or 5A. Cells were labeled with [3H]adenine and treated with or without PTX (100 ng/ml) overnight. cAMP accumulation was assayed in response to the treatment with 100 nM fMLP for 1 h. * cAMP accumulation was significantly increased as compared with their corresponding basal value; Tukey-Kramer's test, p < 0.05.

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Journal of Molecular Signaling

ISSN: 1750-2187