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Figure 5 | Journal of Molecular Signaling

Figure 5

From: Mutations on the Switch III region and the alpha3 helix of Galpha16 differentially affect receptor coupling and regulation of downstream effectors

Figure 5

Coupling of alanine mutants of Gα 16 to different categories of G protein-coupled receptors. Individual GPCRs were coexpressed with Gα16, 2A, 3A or 5A in COS-7 cells. Transfected cells were labeled with [3H]myo-inositol and treated with PTX overnight. Cells were then treated with the appropriate receptor agonists (1 μM CHA for A1R, 100 nM C5a for C5aR, 100 nM fMLP for fMLPR, 10 μM adenosine for both A2AR and A2BR, 10 μM dopamine for D1R) for an hour before extraction of accumulated labeled IP. *Agonist significantly stimulated IP production as compared to their corresponding untreated counterparts; Tukey-Kramer's test, p < 0.05. ‡ Agonist-induced responses in mutant-expressing cells were significantly lower than that obtained with Gα16; Tukey-Kramer's test, p < 0.05.

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Journal of Molecular Signaling

ISSN: 1750-2187