Table 4 Primer sequences for RT-PCR analysis of human Frizzled (hFz) and LRP genes
From: Wnt and Frizzled RNA expression in human mesenchymal and embryonic (H7) stem cells
Gene | Accession No. | Sequence (5' to 3') | Amplicon (bp) |
|---|---|---|---|
hFz1 | NM_003505 | (F) gtgagccgaccaaggtgtat (R) cagccggacaagaagatgat | 184 |
hFz2 | NM_001466 | (F) gcgtcttctccgtgctctac (R) ctgttggtgaggcgagtgta | 286 |
hFz3 | NM_017412 | (F) tgagtgttcgaagctctatgg (R) atcacgcacatgcagaaaag | 229 |
hFz4 | NM_012193 | (F) aacctcggctacaacgtgac (R) gttgtggtcgttctgtggtg | 303 |
hFz5 | NM_003468 | (F) aacctcggctacaacgtgac (R) gttgtggtcgttctgtggtgc | 322 |
hFz6 | NM_003506 | (F) attttggtgtccaaggcatc (R) tattgcaggctgtgctatcg | 311 |
hFz7 | NM_003507 | (F) gtgcagtgttctcccgaact (R) gaacggtaaagagcgtcgag | 544 |
hFz8 | NM_031866 | (F) tcttgtcgctcacatggttc (R) tgtagagcacggtgaacagg | 375 |
hFz9 | NM_003508 | (F) cgctggtcttcctactgctc (R) agaagaccccgatcttgacc | 414 |
hFz10 | NM_007197 | (F) gcggtgaagaccatcctg (R) gcacggtgtacagcacagag | 276 |
hLRP5 | NM_002335 | (F) accggaaccacgtcacag (R) gggtggataggggtctgagt | 305 |
hLRP6 | NM_002336 | (F) aggcacttacttccctgcaa (R) gggcacaggttctgaatcat | 274 |