Table 3 Primer sequences for RT-PCR analysis of human Wnt (hWnt) genes
From: Wnt and Frizzled RNA expression in human mesenchymal and embryonic (H7) stem cells
Gene | Accession No. | Sequence (5' to 3') | Amplicon (bp) |
|---|---|---|---|
hWnt1 | NM_005430 | (F) gcgtctgatacgccaaaatc (R) ggattcgatggaaccttctg | 244 |
hWnt2 | NM_003391 | (F) tagtcgggaatctgcctttg (R) ttcctttcctttgcatccac | 221 |
hWnt2b | NM_004185 | (F) ctcatcagcaggggtagtcc (R) aaaacggacaccgtagtgga | 159 |
hWnt3 | NM_030753 | (F) acgagaactcccccaacttt (R) gatgcagtggcatttttcct | 170 |
hWnt3a | NM_033131 | (F) tgttgggccacagtattcct (R) atgagcgtgtcactgcaaag | 302 |
hWnt4 | NM_030761 | (F) ccttcgtgtacgccatctct (R) gcctcattgttgtggaggtt | 250 |
hWnt5a | NM_003392 | (F) ccacatgcagtacatcggag (R) cactctcgtaggagcccttg | 378 |
hWnt5b | NM_032642 | (F) gtgcagagacccgagatgtt (R) caggctacgtctgccatctt | 550 |
hWnt6 | NM_006522 | (F) ggttatggaccctaccagca (R) aatgtcctgttgcaggatgc | 208 |
hWnt7a | NM_004625 | (F) agtacaacgaggccgttcac (R) gcacgtgttgcacttgacat | 326 |
hWnt7b | NM_058238 | (F) aagctcggagcactgtcatc (R) ccctcggcttggttgtagta | 374 |
hWnt8a | NM_058244 | (F) tggggaacctgtttatgctc (R) ccctcggcttggttgtagta | 456 |
hWnt8b | NM_003393 | (F) ctggtccaaaggcttacctg (R) tgagtgctgcgtggacttc | 557 |
hWnt9a | NM_003395 | (F) gacggtcaagcaaggatctg (R) tgctctcgcagttcttctca | 411 |
hWnt9b | NM_003396 | (F) ctgcttgagtgccagtttca (R) cgagtcatagcgcagtttca | 477 |
hWnt10a | NM_025216 | (F) aatgccaacaccaattcagg (R) caactcggttgttgtgaagc | 464 |
hWnt10b | NM_003394 | (F) gcaagagtttcccccactct (R) gattgcggttgtgggtatc | 367 |
hWnt11 | NM_004626 | (F) ttgcttgacctggagagagg (R) gacgagttccgagtccttca | 521 |
hWnt16 | NM_007168 | (F) tgctccgatgatgtccagta (R) acctcctgcaacggacatag | 562 |