Figure 4
From: The neurotransmitter dopamine modulates vascular permeability in the endothelium
Confocal microscopy images of tight junctions in HUVEC. (A) normal ZO-1 in untreated control HUVEC (B) disrupted ZO-1 in VPF/VEGF (10 ng/ml) treated HUVEC (C) dopamine (10 μM) + VPF/VEGF (10 ng/ml) treated HUVEC shows that pre-treatment with dopamine inhibits the ZO-1 disrupting property of VPF/VEGF (D) quinpirole (10 μM) + VPF/VEGF (10 ng/ml) treated HUVEC shows that pre-treatment with quinpirole inhibits the ZO-1 disrupting property of VPF/VEGF. The figures are representative of three separate experiments with similar results. Average intensity of ZO-1 staining for each cell was measured using metamorph software (version 7, Molecular Devices Inc., Toronto, Canada). Scale bar, 20 μm.