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Figure 7 | Journal of Molecular Signaling

Figure 7

From: CK2beta gene silencing increases cell susceptibility to influenza A virus infection resulting in accelerated virus entry and higher viral protein content

Figure 7

siRNA-mediated CK2β gene silencing does not influence viral polymerase activity. Polymerase activity was assayed in a minigenome system, using a viral UTR-driven luciferase reporter gene. A549 cells were transfected with either control or CK2β siRNA for 48 h, then co-transfected with plasmids containing the PB2, PB1, PA, and NP genes plus a luciferase reporter plasmid. Cells not transfected with the PB1 plasmid were used as negative controls. After 24 h, polymerase (luciferase) activity was assayed in cell extracts. Results represent the mean ± SE of 3 independent experiments.

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Journal of Molecular Signaling

ISSN: 1750-2187