Regulation of thymus-dependent and thymus-independent production of immunoglobulin G subclasses by Galpha12 and Galpha13

Table 1 Production of Ig classes and IgG subclasses in WT mice or mice deficient in Gα₁₂ and/or Gα₁₃

	Thymus-dependent Ig production					Thymus-independent Ig production
	WT	Gα₁₂ +/-	Gα₁₂ -/-	Gα₁₃ +/-	Gα₁₂/Gα₁₃+/-	WT	Gα₁₂ +/-	Gα₁₂ -/-	Gα₁₃ +/-	Gα₁₂/Gα₁₃ +/-
IgG	++	++	+	+	+	++++	+++	+	++	+
IgG1	+++	+++	+	++	+	+	+	+	+	+
IgG2a	++	+	+	+	+	+	+	+	+	+
IgG2b	++	++	+	+	+	++++	+++	+	++	+
IgG3	t	t	t	t	t	+	+	+	+	++
IgM	+	+	+	++	+	+	+	+	+	+
IgA	t	t	t	t	t	t	t	t	t	t
IgE	t	t	t	t	t	Not detected

The relative Ig production was defined as followings: -, OD₄₅₀ < 0.15; +, OD₄₅₀ 0.15–0.4; ++, OD₄₅₀ 0.4–0.75; +++, OD₄₅₀ 0.75–1.0; and ++++, OD₄₅₀ > 1.0. The absorbance at 450 nm (OD₄₅₀) was measured using ELISA assays. Number of animals in each treatment group = 10