Figure 1

Antigen-specific Ig production in mice immunized with antigens. A) OVA-specific Ig production in mice immunized with OVA. Wild type (WT) or Gα₁₂/Gα₁₃ knockout (KO) mice were i.p injected with OVA on day 1 and day 14, respectively, and challenged by nebulizing 1% OVA solution for 3 consecutive days (days 26–28). Secondary immune responses for OVA-specific total IgG or IgM class, or OVA-specific IgG subclasses in sera were assessed on day 29. B) Ig production in mice injected with a single dose of TNP-LPS. Wild type (WT) or Gα₁₂/Gα₁₃ knockout (KO) mice were injected with TNP-LPS (25 μg/mouse) on day 1. After 2 weeks, TNP-LPS-specific total IgG or IgM class, or TNP-LPS-specific IgG subclasses were assayed in the sera. Preimmune sera obtained on day 0 were used as controls. Number of animals in each treatment group = 10. Data represent means ± S.E.M. (significant compared to the respective Ig in WT mice *p < 0.05, **p < 0.01). OVA, ovalbumin; TNP, trinitrophenyl; LPS, lipopolysaccharide.