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Figure 7 | Journal of Molecular Signaling

Figure 7

From: SFRP-4 abrogates Wnt-3a-induced β-catenin and Akt/PKB signalling and reverses a Wnt-3a-imposed inhibition of in vitro mammary differentiation

Figure 7

The Wnt-3a-imposed abrogation of differentiated response to lactogenic hormones was mitigated in pBabeSFRP-4-infected Line 31E cells. Using Northern blot methods, the expression of β-casein was investigated after stimulation of cultures with Wnt-3a and/or different combinations of growth factors and lactogenic hormones. Lines 31E +30F co-cultures containing either pBabeVector or pBabeSFRP-4-infected Line 31E cells were compared. Most notable is that Wnt-3a is capable of completely suppressing the differentiation to β-casein expression stimulated by dexamethazone-insulin-prolactin (DIP). However, co-cultures containing Line 31E cells infected with SFRP-4 show definite β-casein induction in spite of Wnt-3a treatment. Ribosomal RNA was stained with acridine orange as a loading control.

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Journal of Molecular Signaling

ISSN: 1750-2187