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Figure 1 | Journal of Molecular Signaling

Figure 1

From: SFRP-4 abrogates Wnt-3a-induced β-catenin and Akt/PKB signalling and reverses a Wnt-3a-imposed inhibition of in vitro mammary differentiation

Figure 1

Dvl-3 as a marker of Wnt pathway activation. Line 30F (left panel) or co-cultures of Lines 31E + 30F (right panel) stimulated with ectopic Wnt-3a respond with hyperphosphorylation of Dvl-3. Following treatment with Wnt-3a for the indicated times, cells were lysed with Triton X-100 Lysis Buffer and cell extracts were subjected to Western blot analysis. Hyperphosphorylation of Dvl-3 is evident by a consistent relative increase in the intensity of the upper band of the doublet. Alkaline phosphatase treatment caused the loss of the upper band (data not shown).

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Journal of Molecular Signaling

ISSN: 1750-2187