Figure 5

Phosphorylation of FAK substrates in vivo. CE cells transfected with empty RCAS vector (mock) or RCAS encoding wild type FAK or FAK mutants were left untreated (-) or treated with vanadate overnight prior to lysis (+). A) p130CAS was immunoprecipitated from 1 mg lysate and the immunoprecipitated protein was immunoblotted for phosphotyrosine (upper panel) and p130CAS (bottom panel). B) Paxillin was immunoprecipitated from 1 mg lysate and the immunoprecipitated protein was immunoblotted for phosphotyrosine (top panel) and paxillin (bottom panel).