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Figure 4 | Journal of Molecular Signaling

Figure 4

From: PI3 K/Akt/mTOR-mediated translational control regulates proliferation and differentiation of lineage-restricted RoSH stem cell lines

Figure 4

Phosphorylation of mTOR targets and expression of endothelial markers during differentiation and rapamycin treatment. At 0, 6, 24 and 48 hours after RoSH2 cells were induced to differentiate by plating on matrigel-coated plates or treated with 50 ηM rapamycin, the cells were harvested for western blot analysis and probed for a, d) endothelial markers, Tie-2 and Flk-1; b, c) Eif4ebp1 and phosphorylated Rps6kb1. Tsc2 protein was used as an internal control for loading between lanes and between blots.

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Journal of Molecular Signaling

ISSN: 1750-2187