Figure 1

Growth and cell cycle regulation during endothelial differentiation. a) Rate of cell proliferation. RoSH2 cells were labeled with CFDA, a cell-permeable fluorescent dye, and then cultured for 24 hours. They were then plated on gelatin-coated plates under non-differentiating condition or on matrigel-coated plate under differentiating condition. At 0, 24, 48 and 72 after plating, cells were harvested, median cellular fluorescence was measured by flow cytometry and the number of cell divisions was calculated as a function of the loss in fluorescence for every twenty-four hour; b) the cell numbers were counted and normalized against cell count at time 0. Cell number at time 0 was designated as one.