Figure 4

Dynamics of endogenous Sigma-1 receptor and Talin appearence in the FAC in CHO-K1 cells under various treatment conditions. All the compounds indicated were added to cells in complete 10% FBS supplemented DMEM for 30 min at a concentration of 10 μM except phalloidin (50 nM). Morphometrical quantitation of the amount of the sigma-1 receptor or talin in the FAC was performed with the Axiovision 4.3 software. The total amount of staining (in pixels) for each channel was quantitated for each image and divided by the number of cells (detected by DAPI staining of nuclei). 101–155 cells were measured per each condition.